Uruguayan giants Penarol are in danger of missing the knockout stages of the Copa Libertadores after losing on Tuesday.
Penarol – who have won the competition five times – dropped their second Group 4 match on the trot, losing 3-1 against Velez Sarsfield of Argentina.
They still sit second in the group, but only by goals scored, with Ecuadorian side Emelec equal on points and goal difference in third.
The latest defeat came despite Fabian Estoyanoff's 24th-minute penalty, which gave Penarol the lead.
Velez Sarsfield were playing in front of their home fans though, and they quickly mustered a response when Penarol goalkeeper Enrique Bologna scored an own goal.
Federico Insua then bagged a 75th-minute penalty before a late Jonathan Copete strike wrapped up the win for the Argentines.
Velez Sarsfield now top Group 4 with three wins from four matches.
In Group 2, Sporting Cristal claimed a 1-1 draw with Libertad of Paraguay.
Pablo Velazquez fired Libertad – who lead the group with eight points from four matches – ahead early in the second half before the Peruvian side levelled through Irven Avila on 74 minutes.
In other matches, last year's semi-finalists Universidad Chile slumped to a 2-0 home loss against Newell's Old Boys of Argentina.
Universidad still top the group but the win was crucial for Newell's Old Boys, who had failed to pick up a point in their two previous Group 7 matches.
Maximiliano Rodriguez and Martin Tonso scored for the winners.
And in Group 8, Venezuelan side Caracas came from behind to beat Brazilian outfit Gremio 2-1.