Hoffenheim will travel to the Westfalenstadion on Saturday looking to preserve their Bundesliga status.
A win for Markus Gisdol's men, coupled with favourable results elsewhere, could see second-bottom Hoffenheim finish in either the relegation play-off spot, or completely out of the relegation places.
However, Hoffenheim are in poor form, having won just twice in six games, and come into the match with off the back of a 4-1 defeat against Hamburg.
They also face a Dortmund side who are guaranteed second place in this year's Bundesliga and are unbeaten in eight league matches.
Jurgen Klopp's men were last beaten on March 9 by rivals Schalke but since then they have been in excellent form, even booking a place in the UEFA Champions League final next Saturday against Bundesliga champions Bayern Munich.
One crumb of comfort for Hoffenheim is that Dortmund were somewhat fortunate to draw 3-3 last time out against Werder Bremen, having been 3-1 down, while Klopp may choose to rest players ahead of the final at Wembley.
Klopp said in his press conference on Thursday that while Dortmund wanted to win the game, it was not as important to them as it was for their opponents.
"Their goal is a more important one obviously compared to ours," he said.
"Nevertheless we want to take up pace, because last week we didn't really keep our pace. We came back within the game. But before that we had got off to a bad start. So this is something we want to change this time.
"We have a different approach against Hoffenheim (to the UEFA Champions League final). One is a final and the other is a Bundesliga game.
"I usually try to make the players keen on games, but – let me put it this way – this time around there won't be a bonus for the team. It is what it is, so it will be important and also intense."